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Image mixer while installing olympus master

#Image mixer while installing olympus master serial
#Image mixer while installing olympus master manual

Create a 3D model of a structure: Select an area_list>right click>Show in 3D>Choose a value between 1 and 100.If you have an old version of Fiji with Java 6 (note: a version of Fiji coupled with Java 6 is available on Fiji website for Windows and Linux users):.The process of exporting the 3D models depends on your version of Fiji. Select “Z space” in the TrakEM2 canvas and use the brush to draw on the images.To create a second object, simply drag the “area_list” from “Template” column to the folder suited under “Project Objects”. Drag “anything” and “area_list” into the project folder under Project Objects column. In the node window, add a new “area_list” to “anything” under “Template” column.Create an area_list to trace your object.

#Image mixer while installing olympus master manual

Select the type of alignment – s ee the TrakEM2 user manual to know which type of alignment is appropriate.Align a stack (right-click in the canvas>align).Set the pixel size in microns! Blender will only take the value and not the unit.Set the pixel size to have a correct slice thickness (Image>Properties…).To reset the slice thickness, right-click on the image folder and select “Reset layer Z and thickness”.To set the slice thickness later, select all the images of the stack in the TrakEM2 window under “Layers” column, right click and select “Scale Z and thickness”.Example: 10nm slice thickness / 5 nm per pixel = 2 (that means TrakEM2 has to put 2 pixels between each slice to reach the 10nm).This is calculated by dividing the slice thickness (in nm) by the pixel size (resolution) – set the slice thickness to this value. Right-click in the black canvas and select Import>Stack and pick the opened stack. 6 Picture PVW, PIP When the switcher is connected with an external expansion screen, it can preview real-time monitoring on 6 pictures, including PGM, PST and 4.

#Image mixer while installing olympus master serial

Once the destination has been chosen, two windows will open, a project window showing the node structure of your reconstruction project, and a display window in which the serial images with appear, with a tool box and list on the left hand side (see image below).

Choose a folder where the TrakEM2 project will be saved.

Open a new TrakEM2 canvas (File>New>TrakEM2 (blank).

Tick the box ‘Convert to 8 bit Grayscale’ and “Use virtual stack” (to save memory).

Select the folder where the images are situated and click on import.

Import an image sequence (File>Import>Image sequence…).

Open the software Fiji (can be downloaded here).

This user guide provides step by step instructions to load your image stack into Fiji and TrakEM2, align and trace your samples, export the 2D traced objects as 3D models, and load these 3D models in Blender